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The Antler-shaped Fruiting Body of Ganoderma lucidum Suppresses LPS/IFN-γ-induced Inflammation via Inhibiting MAPK/AP-1 Signal Pathway
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/348634
The purpose of this study was to investigate whether or not the antler-shaped fruiting body of Ganoderma lucidum (GL) has an anti-inflammatory effect on lipopolysaccharide (LPS)/interferon-γ (IFN-γ)-activated RAW 264.7 macrophage-like cells. To evaluate the anti-inflammatory effects of GL, we examined the inflammatory mediators such as the production of nitric oxide (NO) and the expression of mitogen-activated protein kinase (MAPK), activator protein 1 (AP-1), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and interleukin-6 (IL-6). LPS/IFN-γ-induced cellular NO production was significantly decreased in GL-treated RAW 264.7 cells. Moreover, Western blotting analysis results demonstrated that reduced protein expression of MAPK families (such as extracellular signal-regulated kinase, c-Jun amino-terminal kinase, and p38 MAPK) and AP-1-targeting inflammatory enzymes (iNOS, COX-2, IL-1β, and IL-6). These results indicated that GL modulates the MAPK/AP-1 signal pathway in inflammatory process. In conclusion, the present study provides important evidence that GL can potentially be used to reduce LPS/IFN-γ-induced inflammatory response by inhibiting the MAPK/AP-1 signaling pathways.
