Lentinula edodes, a member of the Pleurotaceae, is a blood pressure and cholesterol lowering effect. Recently, research the reduction of irritation and the enhancement of function have been cultivation of natural resources using mushroom mycelium. For the development of food fermented with L. edodes mycelium of natural medicinal herb remnants, determined to be 20%, and the product were used after lyophilization. In this study, we were analyzed the contents of β-glucan and amino acids of fermented herb remnants by L. edoeds mycelials. β -Glucan is a type of polysaccharide that has immune-enhancing and is known to inhibit cancer cells. The content of β-glucan and total amino acids of fermented herb remnants by L. edoeds mycelials were 35.13% and 8,697.36 mg%, respectively. The content of essential amino acid from fermented herb remnants by L. edoeds mycelials was 3,226.85 mg%. Collectively, our findings suggest that fermented herb remnants by L. edoeds mycelials extract could be used as an chemo-preventive material for consumers.