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Characteristics of Antagonistic Bacteria for Biological Control of Button Mushroom Disease by Cladobotryum mycophilum
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/348656
Cladobotryum mycophilum is the causal agent of cobweb disease of commercial mushrooms. Symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps and progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing. Two gram-positive bacterium were isolated from mushroom media that markedly showed the antagonistic activity against C. mycophilum.. These isolates were identified as Bacillus altitudinis and Bacillus subtilis by analysis of the cultural, morphological and physiological characteristics, and analysis of the 16S rDNA. The isolated bacteria is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacteria cell was sufficient for inhibition in vitro for C. mycophilum. Control efficacy of browning disease of Bacillus altitudinis treatment was 78% on Agaricus bisporus. The optimal culture medium was determined as follows: 3% Soluble startch, 10% Soytone, 1% (NH4)2HPO4, 1 mmol KCl, and 0.5% L-asparagin at pH 6.0 at 30°C. Control efficacy of browning disease of Bacillus subtilis treatment was 71% on Agaricus bisporus. The optimal culture medium was determined as follows: 1.5% Xylose, 2% Soytone, 1% NH4H2PO4, 7 mmol CaCl2, and 0.5% Histidine at pH 6.0 at 25°C. Accordingly, the suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by C. mycophilum.
