Tremella fuciformis has about 40 species worldwide, and is widely distributed in the subtropical, tropical, and temperate regions. Tremella fuciformis is known to have been eaten by poppy in Chinese classics and has been a symbol of beauty since ancient times. In fact, it has been known that the extract of the Tremella fuciformis induces inhibition of melanin formation of the cells to have a skin whitening effect and to prevent wrinkles caused by UV stimulation damage, thereby improving wrinkles. Tremella fuciformis was first cultivated in Sichuan province, China, and is now cultivated in China and Southeast Asia. China has succeeded in large-scale artificial cultivation of Tremella fuciformis, but there is no research on mass artificial cultivation in Korea. Therefore, this study was carried out to develop standard cultivation technique for mass production of Tremella fuciformis. In order to select the optimal medium for Tremella fuciformis, five kinds of medium such as PDA, MCM, MEA, YM and CDA were used. Tremella fuciformis showed the best growth on PDA medium, and Annulohypoxylon stygium showed the best growth on MCM. The growth of Tremella fuciformis was good at pH 5 , and Annulohypoxylon stygium showed the best growth at pH 6. The optimum temperature for Tremella fuciformis and Annulohypoxylon stygium was 25 °C. Carbon source and nitrogen source were selected to establish optimal culture conditions.