A demand for new mushrooms in the market has given impetus to explore new sources of edible mushrooms. Lepista nuda has an international reputation as an excellent edible species. The combination of its lilac color, solid fleshy structure, good shelf life, and delicious flavor makes it attractive to the market. Therefore the development of commercial cultivation techniques for L. nuda is highly desirable. This study was carried out to develop commercial cultivation techniques of L. nuda for the wild mushroom industry. For commercial cultivation of L. nuda it is required to research into environmental and physical parameters and to develop the new varieties with good productivity and short cultivation period. Twelve of L. nuda were provided from National Agrobiodiversity Center, Korea in 2012. They were investigated mycelia1 growth and formation of fruiting bodies on Agaricus compost. Only five among twelve, KACC51942, KACC52012, KACC52212, KACC52227, and KACC52139, succeeded in initiation and development of fruiting bodies. Spore collection and isolation of monokaryons were obtained from four except KACC52012. We selected four of L. nuda, KACC51942, KACC52212, KACC52227, and KACC52139, as crossing parents to develop the new varieties to shorten cultivation period. They showed different mycelial growth on agar and specific shape and color of fruiting body on button mushroom bed.