The Pleurotus tuoliensis is a mushroom named Chinese ‘Bai-Ling-Gu’ from species of Pleurotus nebrodensis. we are mated for selection of cytoplasmic hybrid by mitochondria microsatellite marker and the method of Mon-Mon mating between monokaryotic strains derived from Pleurotus ferula ASI 0629 (Beesan No.1) and Pleurotus tuoliensis ASI 0663 (Baekryung 20). The cytoplasmic hybrids were identified 8 strains contained nuclear DNA bands of 'Baekryung 20' and mitochondrial DNA of monokaryon strains derived from Pleurotus ferula ASI 0629 (beesan No.1). The KiMB-Plft-15-81 was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Baekwhang'. The 'Baekwhang' cab be grown without an extra after-ripening period, can utilize bottle cultivation of Pleurotus eryngii. And also the ‘Seolwon’ is similar to the existing Pleurotus tuoliensis in shape and physiological characteristics, formed high stipe. We therefore expect that this new strains will substitutional goods of Pleurotus eryngii.

• Pyung Gyun Shin(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
• Min-Ji Oh(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
• Yeun-Lee Oh(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
• Kab Yeul Jang(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
• Won Sik Kong(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
• Young Bok Yoo(Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)