This study was aimed to develop a novel qualitative multiplex polymerase chain reaction (PCR) for simultaneous detection of genetically modified (GM) soy and maize within a single reaction. The specific primers designed to detect four respective GM events (A2704-12, MON88017, Bt11, and MON863) were included in the tetraplex PCR system. Each of PCR products for four GM events could be distinguished by agarose gel based on their different lengths. The specificity and reproducibility of this multiplex PCR were evaluated. This multiplex PCR consistently amplified only a fragment corresponding to a specific inserted gene in each of the four GM events and also amplified all four of the PCR products in the simulated GM mixture. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM soy and maize in a single reaction.

ABSTRACT
 Materials and Methods
  Materials
  Preparation of test samples and DNA extraction
  Oligonucleotide primers
  Analysis of PCR products
 Results and Discussion
 Acknowledgment
 References

• Kyoung Sik Park(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Mi-Gyeong Kim(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Donggil Leem(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Taehyung Yoon(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Ki-Mi No(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Jin Hong(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Eun Mi Kwon(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Ae Rie Moon(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration)
• Jayoung Jeong(Nutrition and Functional Food Research Team, National Institute of Food & Drug Safety Evaluation, Korea Food & Drug Administration) Correspondence to