The aim of study was to investigate the virulence profile of Escherichia coli O157:H7 bacteriophages isolated from sewage and livestock stools. Among 23 E. coli O157:H7 bacteriophages, 14 strains were isolated from sewage and 9 were from animal stools collected from 10 livestock farms in Korea. For each bacteriophage DNA sample, the presence of stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, and cnf was examined by polymerase chain reaction. The detection rate of eae, stx2, estI, astA, and ial was 100%, 69.6%, 13.0%, 13.0%, 8.7%, respectively. While all E. coli O157:H7 bacteriophages isolated from stools carried eae+stx2, stx2+eae, eae+astA, eae, stx2+eae+estI, eae+estI, stx2+eae+ial, and eae+ial were observed in bacteriophages isolated from sewage. As several plasmid-carrying virulence factors (estI, astA, and ial) were found in E. coli O157:H7 bacteriophages obtained from sewage and stools, the microbial safety of bacteriophages should be investigated in further study.

ABSTRACT
 재료 및 방법
  공시균주 및 박테리오파지 분리 가검물
  E. coli O157:H7 박테리오파지 분리
  E. coli O157:H7 박테리오파지의 병원성인자 검출
 결과 및 고찰
 참고문헌

• 서지나(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Jina Seo
• 서동주(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Dong Joo Seo
• 이민화(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Min Hwa Lee
• 전수빈(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Su Been Jeon
• 오혜진(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Hyejin Oh
• 오미화(농촌진흥청 국립축산과학원, National Institute of Animal Science, Rural Development Administration) | Mi Hwa Oh
• 최창순(중앙대학교 식품공학부, School of Food Science and Technology, Chung-Ang University) | Changsun Choi Correspondence to