Allergic diseases have increased rapidly over the past decades, affecting an estimated 20~30% of the population in developed countries. In this study, we investigated whether or not a typical costal sand dune plant Carex pumila (CPE) suppresses the activation of mast cells and IgE-mediated allergic response in vitro and in vivo. As the results, the extract of Carex pumila inhibited antigen-stimulated degranulation in RBL-2H3 cells and Bone marrow-derived mast cells (BMMCs), and IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. CPE also suppressed the production of pro-inflammatory cytokines, TNF-α and IL-4, in antigen-stimulated mast cells. As its mechanism of action, CPE inhibited the activation of Syk in FcεRI-mediated signalling pathway, and that of LAT, a downstream adaptor molecule of Syk, in a dose-dependent manner. CPE also suppressed the activation of mitogenactivated protein (MAP) kinases, p38, ERK1/2, JNK, and Akt. Altogether, CPE inhibited mast cell activation and IgEmediated allergic response by antigen through suppressing the activation of Syk. These results suggest that CPE may be useful for the treatment of allergic diseases.

ABSTRACT
 실험재료 및 실험방법
  실험재료
  실험동물
  비만세포의 분리 및 배양
  좀보리사초의 메탄올 추출물 구입
  비만세포의 탈과립 측정
  RT-PCR (Reverse Transcriptase-Polymerase ChainReaction)
  Immunoblot analysis
  수동 피부 감작 알레르기 동물모델
  통계학적 분석
 결 과
  좀보리사초 추출물의 RBL-2H3 세포와 BMMC의 탈과립에대한 억제 효과 및 가역적 효과
  좀보리사초 추출물의 항원에 의해 유도된 TNK-α 및 IL-4생성에 대한 억제 효과
  좀보리사초 추출물의비만세포에서 Syk 및 하위 신호전달단백질 활성화 억제 효과
  동물모델에서 좀보리사초 추출물의 알레르기 억제 효과
 고 찰
 참고문헌

• 임한나(덕성여자대학교 약학대학, College of Pharmacy, Duksung Women’s University) | Hannah Lim
• 김영미(덕성여자대학교 약학대학, College of Pharmacy, Duksung Women’s University) | Young Mi Kim Correspondence to