The safety of a new natural plant composition (ADP) was assessed on the genotoxicity study and 14-day repeat dose toxicity study. ADP contains a mixed water extract obtained from the mixture of Phellodendron cortex (Phellodendron amurense) and Anemarrhena rhizoma (Anemarrhena asphodeloides), and poses the contractile properties mediated by alpha-adrenoceptor of the prostate and urethra as well as antioxidant and anti-inflammatory properties. In order to evaluate genetic safety, in vivo micronucleus test was performed in ICR mice orally administered with three dose levels of 1250, 2500, 5000 mg/kg body weight, and vehicle and positive control. In the 14 days study, Sprague-Dawley rats were treated with ADP at the dose levels of 500, 1000, 2000 mg/kg once a day, and clinical signs, body weights, hematology, serum biochemistry, necropsy findings and organ weights were monitored and examined. In experimental results, ADP treatment, compared with vehicle control, did not induce the micronucleated erythrocytes from mouse bone marrow. In the 14 days study, any significant and toxicological differences in all measurements of parameters were not observed in ADP treatment groups of animals, compared with vehicle treatment. The No-Observed-Adverse-Effect-Level (NOAEL) of ADP in the 14 days study was determined to be greater than 2000 mg/kg/day in both sexes.

ABSTRACT
 실험재료 및 방법
  실험 시료 및 재료
  실험 동물 및 관리
  마우스를 이용한 소핵시험
  14일 반복 경구투여 독성시험
  통계학적 분석
 결과 및 고찰
  마우스를 이용한 소핵시험
  14일 반복 경구투여 독성시험
 결 론
 참고문헌

• 정영신(호서대학교 생명공학과, Department of Biotechnology, Hoseo University) | Young-Shin Chung Correspondence to
• 박철범(호서대학교 융합기술원, Hoseo Fusion Technology Research Center, Hoseo University) | Cheol-beom Park