Petroleum energy is the major source of the world energy market, and its massive usage, and the corresponding extreme environmental pollution, imposes a serious threat on the ecological cycles. By screening oil-contaminated soil, we isolated, identified, and characterized a novel strain that represents a considerable diesel-degrading potentiality; the Bacillus aryabhattai DA2 strain is registered in the NCBI with the accession number MG571630, and it possesses an efficient tributyrin-degrading capacity. The optimal condition for diesel degradation by DA2 strain was observed at pH between 7-8 and at the temperature of 30°C. The strain is resistant to salt as well as the antibiotics like ampicillin and streptomycin. These results indicate B. aryabhattai is one of the potential candidates for the remediation of the diesel-contaminated sites.

Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  1. Isolation of diesel degrading bacteria in thecrude oil
  2. Lipolytic activity assay
  3. Antibiotic resistivity performance test
  4. Salt resistivity performance test
  5. Selection, identification and phylogenetic treeconstruction
  6. Determination of optimal conditions for dieseldegradation
  7. Residual diesel component analysis
  8. Enzyme activity assay
  9. Biochemical assay
  10. Statistical analysis
 RESULTS AND DISCUSSION
  1. Isolation, selection, and identification ofoil-degrading microorganisms
  2. Determination of optimal stage for degradation
  3. Antibiotic and salinity tests
  4. Determination of lypolytic activity
  5. Biochemical characteristics
  6. Catechol 1,2-dioxygenase activities
 CONCLUSION
 REFERENCES

• Sang-Jun Kim(Department of Natural Sciences, Naval Academy)
• Arjun Adhikari(School of Applied Biosciences, Kyungpook National University)
• Ko-Eun Lee(School of Applied Biosciences, Kyungpook National University)
• Gil-Jae Joo(Institute of Agricultural Science and Technology, Kyungpook National University) Corresponding author