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Aquaporin 5 expression is regulated by estrogen receptor α in mouse oviductal epithelium
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/355137
Aquaporin5 (AQP5), a water channel plays an important role in the fluid homeostasis and cell volume control in epithelial cells. In an effort to understand fluid homeostasis in the oviduct, tissue specific expression of AQP 5 was examined together with hormonal regulation of AQP5 in the mouse oviduct. To understand the oviductal fluid homeostasis and its regulation by sex steroids, We examined AQP5 expression in mouse oviduct during developmental stage and estrous cycle, and in estrogen receptor α (ERα) knockout mice oviduct. In immature mouse oviduct, expression of AQP5 expression was examined after stimulation with gonadotropins. The effect of ERα agonist (PPT) and ERβ agonist (DPN) on the oviductal expression of AQP5 was examined in ovariectomized mouse. All samples were subjected to realtime-PCR and immunohistochemistry analysis. In oviduct epithelium, AQP5 was largely found in the apicolateral membrane and cytoplasm of ERα-positive non-ciliated cells but weakly expressed in the ciliated cells. Interstitial cells, muscle cells and blood vessels were also weakly positive for AQP5 immunoreactivity. In cyclic female mice oviductal AQP5 mRNA levels were the highest at estrous. In immature mouse oviduct AQP5 mRNA and epithelial immunoreactivity were increased by PMSG, and followed by a decrease after hCG. In ERα KO mice oviduct, AQP5 mRNA levels were significantly lower than those of WT females at diestrous stage. In immature and OVX mouse oviducts, AQP5 mRNA and epithelial immunoreactivity were significantly increased by E2 and PPT. Together, our results suggest the pivotal role of AQP5 in fluid secretion and absorption of water in non-ciliated cells in oviduct. AQP5 gene is tightly activated by estrogen – ERα signaling in non-ciliated cells in oviductal epithelium, mediating the effect of estrogen on gamete transport, fertilization and early embryo development via regulating the fluid homeostasis in oviduct.
