Objective : To investigate the effects of Simvastatin and Methylprednisolone on ovarian tissue cryopreservation and transplantation using mouse models. Methods : The mice were randomly distributed into 1 control and 3 experimental groups. The B6D2F1 mice were given oral Simvastatin (5 mg/kg), intravenous Methylprednisolone (15 mg/kg), or a combination of both at 2 hours before ovariectomy. Same volume of normal saline was given perorally in the control group at 2 hours before ovariectomy. The ovarian tissues were vitrified accrording to our protocols. The vitrified ovaries were warmed 1 week later and auto-transplanted under bilateral kidney capsules. The ovaries and blood sera were collected at 2, 7 or 21 days after transplantation. Histological analysis, TUNEL assay, immuno-histochemistry for CD31, serum AMH level and embryonic development after in vitro fertilization were assessed for evaluation. Results : With regard to the total grade 1 follicle rate, both Simvastatin or Methylprednisolone treated groups were significantly increased at 2, 7 or 21 days after transplantation (except Simvastatin treated group at 7 days). A combination of Simvastatin and Methylprednisolone group was significantly improved in terms of the total G1 follicle rate, apoptotic follicle rate, CD31 positive area and serum AMH after ovarian tissue transplantation. However, there were no statistically difference with respect to the oocyte maturation rate, blastulation rate, and the other embryonic development parameters after in vitro fertilization procedure among the four groups. Conclusion : Our results suggest that combined donor Simvastatin and Methylprednisolone have beneficial effects on the quality and function of transplanted ovarian tissues.

• Jaewang Lee(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Eun Jung Kim(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Hyun Sun Kong(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Hye Won Youm(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
• Jung Ryeol Lee(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Byung Chul Jee(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Chang Suk Suh(Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
• Seok Hyun Kim(Department of Obstetrics and Gynecology, Seoul National University College of Medicine)