논문 상세보기
Genetic distances and variations of geographic hairtail populations
Hairtails (Trichiurus lepturus) are the most popular marine products in Korea because of their taste and nutritional value, and Koreans consume them in large quantities. Hairtail, ecologically important warm water fish species, belonging to family Trichiuridae, widely distributed on the coast of the West Sea, South Sea and Jeju Island in the Korean Peninsula and the several sea areas in China under the natural ecosystem. However, in spite of their economic and scientific consequences, a little information currently exist regarding the physiological and ecological levels only of hairtail species in Korea (Koo et al., 2004). Simply the biological fisheries feature, distribution and migration of hairtail (T. lepturus) in Korean waters were surveyed (Park et al., 2005). Currently, imported hairtail have been altered into endemic hairtail because of high edge. In the present study, to explicate the genetic distances and differences among geographical hairtail populations, we accomplished a clustering analysis of three hairtail populations collected from Atlantic, Korea and Chinese site. Muscle tissues were obtained separately from individuals from Atlantic hairtail population (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The muscle was collected in sterile tubes, immediately placed on dry ice, and stored at －40℃ until the genomic DNA extraction. Genomic DNA was extracted and purified under the conditions described previously (Yoon and Kim, 2004). Seven primers (BION-02, BION-03, BION-04, BION-08, BION-09, BION-13 and BION-17) were shown to generate the shared loci, specific loci, unique shared loci to each population and shared loci by the three populations which could be obviously scored. The degree of variability was calculated by use of the Dice coefficient (F), which is given by the formula: F = 2 nab / (na+nb), where nab is the number of bands shared between the samples a and b, na is the total number of bands for sample a and nb is the total number of bands for sample b (Jeffreys and Morton, 1987; Yoke-Kqueen and Radu, 2006). Euclidean genetic distances within- and between-population were also calculated by complete linkage method with the support of the hierarchical dendrogram program Systat version 13 (SPSS Inc., Chicago, IL, USA). Here, the seven decamer primers BION-02, BION-03, BION-04, BION-08, BION-09, BION-13 and BION-17 were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the three populations. In the present study, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population, 55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number of shared loci by the four populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28 loci, with an average of 4 per primer, were observed in the CHP population. Many researchers studied the sizes of DNA fragments in the PCR profiles of five species of Eastern Pacific abalone (genus Haliotis) (Muchmore et al., 1998), black tiger shrimp (Penaeus monodon) (Tassanakajon et al., 1998), shrimp populations (Yoon and Kim, 2003) and deep sea lobster (Puerulus sewelli) (Park et al., 2005). The hierarchical dendrogram point out three main branches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15 ~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals’ CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantly related to CHINESE no. 19 (genetic distance = 0.430). The genetic distance between the Indian Ocean lobster and the Korean Slipper lobster species ranged between 0.040 and 0.612 (Park et al., 2005). Consequently, PCR analysis generated on the genetic data displayed that the geographic AHP population was widely separated from CHP population. From what has been said above, the potential of genetic analysis to identify diagnostic markers for the identification of three hairtail populations has been demonstrated. Generally speaking, using a variety of arbitrary primers, PCR has been applied to identify polymorphic/specific markers particular to line, species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms (McCormack et al. 2000; Yoon and Kim 2004).
