The ultimate goal of this study is to assess the accumulation and distribution of hydrophobically modified glycol chitosan (HGC) as a degradable nanoparticle in the body. To understand the movement of degradable nanoparticle HGC in the body, we intravenously injected a dose of 20 mg/kg of Cy5.5-labeled HGC with size ranging from 320 to 400 nm into ICR mice, and measured the amount of fluorescence remaining in blood and several organs at various time intervals. In blood, the level of Cy5.5-labeled HGC was the highest at 15 min, then after 30 min it decreased rapidly and reached a plateau form 30 min to 28 days. In the tissue we confirmed the presence of nanoparticles at high levels in the order of kidney>liver>submandibular gland until 28 days after injection. However, we did not find the presence of the particles in the brain or testes. These results will provide basic information on HGC as a drug delivery agent.

Abstract
 서 론
 재료 및 방법
  분해성 나노물질의 선정 및 확보
  실험동물
  일반증상 및 사망률 관찰
  혈액 및 조직내 Cy5.5-labeled HGC의 형광량 측정
  Free Cy5.5 imaging 측정
 결 과
  Cy5.5-labeled HGC의 혈중 동태
  Cy5.5-labeled HGC의 체내 표적장기 및 조직축적
  영상을 이용한 Free Cy5.5의 체내 분포
 고 찰
 REFERENCES

• Jung Su Lee(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
• Hyeong-Jin Ji(Laboratory Animal Research Center, Chungbuk National University)
• Hee-youl Chai(Division of Biosafety Evaulation & Control, KCDC)
• Jae-Hwang Jeong(Department of Biotechnology and Biomedicine, Chungbuk Province College)
• Sang Yoon Nam(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
• Young Won Yun(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
• Beom Jun Lee(College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) Corresponding Author