The efficacy of chemical disinfectants is reduced owing to the inactivation of active ingredients after dilution. This study investigated the effect of time on the efficacy of six different disinfectants, after dilution, against avian influenza virus. When used at the recommended concentration, most disinfectants showed efficacy at a high concentrations in the presence of organic materials immediately after dilution, while sodium dichloroisocyanurate-based products, after dilution, showed reduced efficacy over time at low concentrations in the absence of organic materials. Most disinfectants were neutralized by organic materials; however, this could be compensated for by increasing the product dosage. For successful decontamination in farms, disinfectants should be used at high concentrations in accordance with the manufacturers’ recommendations. Furthermore, the presence of organic materials must be taken into consideration, and diluted disinfectant solution should be prepared no more than a day before use.

INTRODUCTION
 MATERIALS AND METHODS
  Viruses and Virus Propagation
  Disinfectants and Carriers
  Suspension Test
  Statistical Analysis
 RESULTS
 DISCUSSION
 ACKNOWLEDGEMENTS
 REFERENCES

• Yangho Jang(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA))
• Gwangjick Lee(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA))
• Jaeyoung Song(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA))
• Hyobi Kim(Veterinary drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA))
• Byungjun Jang(College of Veterinary medicine, Konkuk University)
• Nonghoon Choe(College of Veterinary medicine, Konkuk University) Corresponding Author