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Efficacy of lacticin NK34 against bovine intramammary infections caused by Staphylococcus aureus and Streptococcus agalactiae KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Lacticin NK34 is a small nisin-like bacteriocin present in the supernatants of an isolate of Lactococcus lactis from jeotgal, a salted and fermented Korean food made with seafood such as shrimp, oysters, and fish. Recently, we demonstrated that a partially purified NK34 is highly effective against various Staphylococcus species in a murine infection model. In this study, the two major bacterial pathogens associated with bovine mastitis, Streptococcus aureus and S. agalactiae, were evaluated for their susceptibility to NK34 in vitro using a standard teat-dip assay as well as in vivo using mastitic cows infected with one of these pathogenic strains. The experimental analyses showed a significant decrease (up to 98 times) in the bacterial numbers between the NK34-treated and -untreated teats. Moreover, a dramatic reduction in somatic cell counts was observed at 3 days post-treatment with 10 ml of NK34 administered directly into the mastitic cows. Neither S. aureus nor S. agalactiae were recovered. Taken together, these results imply that lacticin NK34 is an alternative antimicrobial substitute for the treatment of bovine mastitis, caused especially by either S. aureus or S. agalactiae.

목차
Abstract
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of lacticin NK34
  Preparation of the indicator strains
  Determination of the antimicrobial effect of NK34
  Determination of the antimicrobial effect of NK34 inthe mastitic cows
 RESULTS
  In vitro antimicrobial effect of NK34 determined byteat dip assay
  In vivo antimicrobial effect of NK34 against themastitic cows with S. aureus or S. agalactiae
  Antimicrobial activity of NK34 on other gram-positiveand -negative bacterial pathogens
 DISCUSSION
 REFERENCES
저자
  • Kook Joo Ahn(Department of Microbiology, College of Veterinary Medicine, Seoul National University)
  • Sook Shin(Department of Microbiology, College of Veterinary Medicine, Seoul National University)
  • Hyang-Mi Nam(Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs)
  • Hyun-Dong Paik(Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Jang Won Yoon(Department of Microbiology, College of Veterinary Medicine, Seoul National University, Department of Public Health and Zoonoses, College of Veterinary Medicine, Kangwon National University) Corresponding Author
  • Yong Ho Park(Department of Microbiology, College of Veterinary Medicine, Seoul National University, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs) Corresponding Author