In this study, we evaluated the antioxidant activity and protective effects against oxidative DNA damage of the ethyl acetate fraction from the callus of Abeliophyllum distichum Nakai (ECA). Callus of A. distichum was induced on MS medium containing NAA (1 ㎎/L) and 2,4-D (1 ㎎/L), and a sufficient amount was obtained for the extraction by subculture. Acteoside was analyzed and quantified (0.39 ㎎/g callus) from ECA using the high-performance liquid chromatographyphotodiode array detector method. ECA showed very high antioxidative activity as revealed by DPPH and ABTS scavenging assays. The IC50 values were 12.4 and 6.8 ㎍/㎖, respectively. ECA showed protective effects against oxidative DNA damage evaluated by using ψX-174 RF I plasmid DNA. It also inhibited DNA damage by suppressing the oxidative stress-induced protein and mRNA levels of γ-H2AX and p53 in NIH/3T3 cells. In conclusion, ECA protects against oxidative DNA damage through its powerful antioxidant activity.

Abstract
 Introduction
 Materials and Methods
  Plant materials and reagents
  Callus induction
  Extraction and fractionation
  Identification of acteoside by HPLC-PDA analysis
  DPPH radical scavenging activity
  ABTS radical scavenging activity
  Inhibitory effect of φX-174 RF I plasmid DNA onoxidative DNA damage
  Cell culture
  SDS-PAGE and western blot analysis
  Reverse transcriptase-polymerase chain reaction (RT-PCR)
  Statistical analysis
 Results and Discussion
  Identification of acteoside from ECA
  Antioxidant activities of ECA
  Inhibitory effect of ECA against DNA cleavage byoxidative stress
  Effect of ECA on DNA damage in NIH/3T3 cells inducedby oxidative damage
 References

• Tae Won Jang(Department of Medicinal Plant Resources, Andong National University)
• Jae Ho Park(Department of Medicinal Plant Science, Jungwon University) Corresponding author