Hepatic ischemia-reperfusion injury (HIRI) is linked with high mortality rate. Several agents have been developed so far to reduce the risk of HIRI. In this study, we investigated the effects of combined treatment of Ginko biloba leaves extract and vitamin C (GLEVC) on hepatic ischemia-reperfusion injury. To explore the protective effects of GLEVC on HIRI rats model were tested. After the development of HIRI by using clamping method rats were then randomly divided into four groups. Different doses of GLEVC were administered in HIRI rat model. The level of ALT, AST, SOD and MDA content in serum were detected in HIRI groups. Moreover, the activity of SOD, content of MDA, and GSH in hepatic tissue were also examined. Bcl-2 and Bax protein expression were detected by immunohistochemical staining method. Compared with sham group, GLEVC has the protective effect on the HIRI-induced model. Level of ALT, AST, and MDA in blood were significantly lower in GLEVC group compared with HIRI-induced group. Moreover, SOD activity and GSH were increased in GLEVC group whereas MDA content was reduced by GLEVC treatment. Furthermore, HIRI-induced Bax protein was reduced upon GLEVC treatment, whereas Bcl-2 protein expression was enhanced. These results demonstrate that GLEVC treatment may provide potential ameliorative therapy by reducing damaged signaling mechanism in hepatic ischemia/reperfusion injury model.

Abstract
 Introduction
 Materials and Methods
  Materials
  Animal experiments
  Preparation of hepatic ischemia-reperfusion model
  Specimen collection and testing
  Measurement of biochemical parameters
  Immunohistochemistry
  Statistical analysis
 Results
  Effects of GLEVC injection on ALT and AST level in serum
  Effects of GLEVC on activities of serum SOD, MDA inHIRI rat
  Effects of GLEVC on SOD activity and MDA content intissue of HIRI rats
  Effects of GLEVC on expression of Bax and Bcl-2 in livertissue of HIRI rats
 Discussion
 References

• Guang-Hua Xie(Department of General Surgery, YanBian University Hospital)
• Sun Eun Choi(Department of Cosmetology Science, Nambu University)
• Myung-Jae Mun(Department of Biochemical & Polymer Engineering, Chosun University, Nano Bio Research Center, JBF)
• Jae-Hun Jeong(Department of Food Science & Bio-Technology, Jeonnam State University)
• Kwang-Hyun Park(Department of Oriental Pharmaceutical and Development, Nambu University, Department of Emergency Medical Rescue, Nambu University) Corresponding author