This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The IC50 (concentration causing 50% cell death) value showed the highest on MCF-7 cell (538.39 ㎍/㎖) in 70% ethanol extract, and exhibited significant activity against Hela cell (637.87 ㎍/㎖), Calu-6 cell (728.64 ㎍/㎖). The extract of 70% ethanol at 1,000 ㎍/㎖ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner.

Abstract
 Introduction
 Materials and Methods
  Samples preparation
  Total polyphenol determination
  Total flavonoid determination
  Assay of DPPH radical scavenging rate
  Assay of cytotoxicity on human cancer cell lines
  Assay of cytotoxicity on 3T3-L1 cell
  Data analysis
 Results and Discussion
  Total polyphenol and flavonoid contents
  DPPH radical scavenging activity
  Cytotoxicity on human cancer cell
  Cytotoxicity on 3T3-L1 cell
 References

• Hee-Ock Boo(WELLPHYTO Co. Ltd., BI Center, GIST) Corresponding author
• Jeong-Hun Park(WELLPHYTO Co. Ltd., BI Center, GIST)
• Moon-Soon Lee(Department of Industrial Plant Science & Technology, Chungbuk National University)
• Soo-Jeong Kwon(Faculty of Food Nutrition and Cookery, Woosong Information College)
• Hag-Hyun Kim(Faculty of Food Nutrition and Cookery, Woosong Information College)