For the purpose of reuse the wasted by-products from the skate process to the health functional food or medicinal material, chondroitin sulfate was extracted from the skate cartilage with the method of hydrolysis with protease enzyme, and the contents of chondroitin sulfate and hydrolyzed protein were measured qualitatively and quantitatively. The effects of chondroitin sulfate on body weight or liver weight changes, hepatotoxicity elimination and anti-inflammatory actions were measured from in vivo test with feed-treated mice. From the hydrolytic extraction of skate cartilage with the mixture of 1% alcalase and 1% protease for 4 hours, the extraction yield of chondroitin sulfate was about 32.55%. The content and molecular weight of chondroitin sulfate was 26.63% and 2.85×105 Da., respectively and the content ratio of chondroitin sulfate to protein was measured to 1 to 2.76 with gel permeation chromatography. For the odor component, trimethylamine decreased about 30% but almost not ammonia from chondroitin sulfate with the treatment of activated carbon. From the feeding chondroitin sulfate to mice, the control effect of body and liver weights decrease was measured, anti-inflammatory action and hepatotoxicity elimination action were also measured. From these results, process operation conditions for manufacturing of chondroitin sulfate were suggested.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 실험방법
 3. 결과 및 고찰
  3.1. 홍어 연골과 제조된 콘드로이틴 황산의 특성
  3.2. 제조된 콘드로이틴 황산의 기능성
  3.3. 제조공정 설정
 4. 결 론
 감사의 글
 REFERENCES

• 백장미(영산홍어(주), Yeongsan Skate Co., Ltd.) | Jang-Mi Baek
• 강건희(영산홍어(주), Yeongsan Skate Co., Ltd.) | Keon-Hee Kang
• 김상호(영산홍어(주), Yeongsan Skate Co., Ltd.) | Sang-Ho Kim
• 노정숙(동명대학교 식품영양학과, Department of Food Science & Nutritional, Tongmyong University) | Jeong-Sook Noh
• 정갑섭(동명대학교 식품영양학과, Department of Food Science & Nutritional, Tongmyong University) | Kap-Seop Jeong Corresponding author