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pp.789-798
Lactic acid bacteria (LAB) are industrially important microorganisms for probiotics. The recent widespread application of LAB for preparation of functional food is attributable to the accumulating scientific evidence showing their beneficial effects on human health. In this study, we isolated and characterized plant-derived LAB that show angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities. The selected strain K2 was isolated from Kimchi, and identified as Lactobacillus plantarum by 16S rRNA gene analysis. The strain grew under static and shaking culture systems. They were also able to grow in different culture conditions like 25℃∼37℃ temperature, 4∼10 pH range and ∼6% NaCl concentration. L. plantarum K2 was highly resistant to acid stress; survival rate of the strain at pH 2.5 and 3 were 80% and 91.6%, respectively. The strain K2 also showed high bile resistance to 0.3% bile bovine and 0.3% bile extract with more than 74% of survival rate. The cell grown on MRS agar plate containing bile extract formed opaque precipitate zones around the colonies, indicating they have bile salt hydrolase activity. The strain showed an inhibitory activity against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; antibacterial activity was probably due to the lactic acid. The K2 strain showed relatively higher autoaggregation values, antihypertensive and antioxidant activities. These results suggest that L. plantarum K2 could be not only applied as a pharmabiotic for human health but also is also starter culture applicable to fermentative products.
1. 서 론
2. 재료 및 방법
2.1. 젖산균의 분리 및 동정
2.2. 생육 특성 조사
2.3. 생균제 특성 조사
2.4. 항고혈압 및 항산화 활성 조사
3. 결과 및 고찰
3.1. 실험균주의 분리 및 동정
3.2. 실험균주의 생육 특성
3.3. 실험균주의 생균제 특성
3.4. 실험균주의 항고혈압 및 항산화 활성 조사
4. 결 론
감사의 글
REFERENCES
