Given the growing attention to nature and health in modern society, this study considered the role of a waterscape facility as a key element in a landscape’s influence on users’ physiological and psychological responses. The subjects were 28 university students, and the collected data included systolic and diastolic blood pressure, salivary amylase concentration, semantic differential (SD) scales, and profile of mood states (POMS). As compared to a space without any waterscape element (Type G), relaxation in a space with a waterscape element (Type W) was found to significantly reduce of systolic and diastolic blood pressure and salivary amylase concentration. The SD scale and POMS findings showed that Type W evoked active, bright, dynamic, free, vital, interesting, and cheerful images and improved mood states by enhancing vigor while inhibiting depressive feelings. These findings indicate waterscape facilities can improves users’ mood states and may enhance their health.