Large space structures exhibit different natural vibration characteristics depending on the aspect ratio of structures such as half-open angle. In addition, since the actual large space structure is mostly supported by the lower structure, it is expected that the natural vibration characteristics of the upper structure and the entire structure will vary depending on the lower structure. Therefore, in this study, the natural vibration characteristics of the dome structure are analyzed according to the natural frequency ratio by controlling the stiffness of the substructure. As the natural frequency of the substructure increases, the natural frequency of the whole structure increases similarly to the natural frequency of the upper structure. Vertical vibration modes dominate at 30° and 45°, and horizontal vibration modes dominate at 60° and 90°.