The objective of this study is to investigate the response reducing effect of a seismic isolation system installed between 300m dome and supports under both horizontal and vertical seismic ground motion. The time history analysis is performed to investigate the dynamic behavior of single layer lattice domes with and without a lead rubber bearing seismic isolation system. In order to ensure the seismic performance of lattice domes against strong earthquakes, it is important to investigate the mechanical characteristics of dynamic response. Horizontal and vertical seismic ground motions cause a large asymmetric vertical response of large span domes. One of the most effective methods to reduce the dynamic response is to install a seismic isolation system for observing seismic ground motion at the base of the dome. This paper discusses the dynamic response characteristics of 300m single layer lattice domes supported on a lead rubber seismic isolation device under horizontal and vertical seismic ground motions.