It is important to consider interaction between species in understanding structure and function of the biological community. Current ecological issues such as climate change and habitat loss emphasize the significance of the concept of species interaction in that varying species’ interaction across environmental gradients may lead to altered ecological function and services. However, most community studies have focused on species diversity through analysis of quantitative indices based on species composition and abundance data without considering species interactions in the community. ‘Ecological network analysis’ based on network theory enables exploration of structural and functional properties of ecosystems composed of various species and their interactions. In this paper, network analysis of Cheonggye stream as a case study was presented to promote uses of network analysis on ecological studies in Korea. Cheonggye stream has a simple biological structure with link density of 1.48, connectance 0.07, generality 4.43, and vulnerability 1.94. The ecological network analysis can be used to provide ecological interpretations of domestic long-term monitoring data and can contribute to conserving and managing species diversity in ecosystems.