논문 상세보기
Cytological analysis of pregnancy-associated plasma protein-A expression in porcine neonatal testis KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/357310
- DOIhttps://doi.org/10.12750/JET.2018.33.3.177
pp.177-183
The identification of biomarkers of a living tissues is essentially required to understand specific functions of the cells. In previous study, we reported IGFBP 3 as one of the putative biomarkers, by showing specific expression at porcine spermatogonial stem cells (SSCs) of early stage of porcine testis. In this study, we analyzed the expression of seven members of IGFBP family (IGFBPs) in SSCs and histological expression pattern of pregnancy-associated plasma protein-A (PAPP-A), which plays a role on the growth promoting enzyme by cleavage of IGFBPs in testis of 5 days old pig. RT-PCR analysis showed that IGFBP 1, 2, 3, 4, and 6 were expressed at high level specifically in porcine SSCs compared with whole testis. We performed immunohisotochemical staining of testis sections with PAPP-A and protein gene product 9.5 (PGP9.5) which are the known biomarkers for SSCs. We were not able to find co-expression of PAPP-A and PGP9.5; PAPP-A was expressed only in Sertoli cells and PGP9.5 expression was confirmed in spermatogonium. Additionally, we were able to confirm the GATA4 expression in Sertoli and Leydig cells as a regulator of Sertoli cell function was not detected PGP9.5 expressing cells, indicating indirect evidence of that cytolocalization of PAPP-A expression is limited in Sertoli cells. These results suggested that the PAPP-A expressed in Sertoli cells may play role on regulation of development and differentiation of testicular cells through the IGF axis in neonatal porcine testis.
서 론
재료 및 방법
1. 정소 조직 및 세포 준비
2. 유전자 발현 분석
3. 조직 Hematoxylin & Eosin 염색 분석
4. 조직 면역염색 분석
결 과
1. 돼지 정원줄기세포와 정소에서의 IGFBP family member의 발현 분석
2. 5일령 돼지 정소에서 PGP9.5, GATA4, PAPP-A 발현 분석
3. 5일령 돼지 정소에서 PGP9.5와 GATA4의 발현 분석
4. 5일령 돼지 정소에서 PGP9.5와 PAPP-A의 발현 분석
고 찰
적 요
REFERENCES
