A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  1. Primary cell culture and media supplements
  2. Subculture
  3. Cell proliferation assay
  4. Chromosome analysis
  5. Pluripotency genes expression analysis
  6. Transfection
 RESULTS 
  1. Primary culture
  2. Cell growth curve
  3. Chromosome analysis
  4. Pluripotency genes expression
  5. Transfection
 DISCUSSION 
 REFERENCES

• Ju-Won Kim(Biotechnology Research Division, National Institute of Fisheries Science)
• Bang Geun Oh(Biotechnology Research Division, National Institute of Fisheries Science)
• Julan Kim(Biotechnology Research Division, National Institute of Fisheries Science)
• Dong-Gyun Kim(Biotechnology Research Division, National Institute of Fisheries Science)
• Bo-Hye Nam(Biotechnology Research Division, National Institute of Fisheries Science)
• Young-Ok Kim(Biotechnology Research Division, National Institute of Fisheries Science)
• Jung Youn Park(Biotechnology Research Division, National Institute of Fisheries Science)
• JaeHun Cheong(Dept. of Integrated Biological Science, Pusan National University)
• Hee Jeong Kong(Biotechnology Research Division, National Institute of Fisheries Science) Corresponding Author