논문 상세보기
Anti-inflammatory activity of Rhus chinensis extract on bone marrow cells via decrease of metabolic activity and modulation of the production of inflammation-related cytokines KCI 등재
- 언어KOR
- URLhttps://db.koreascholar.com/Article/Detail/358102
pp.20-25
Many kinds of medicinal herbs have been used to treat inflammation in Oriental medicine. However, there few studies have investigated the anti-inflammatory activity of medicinal herbs. In this study, we used mouse bone marrow cells (BMs) treated with lipopolysaccharide (LPS), a simulator of osteomyelitis, to screen medicinal herbs having anti-inflammatory activity. Specifically, we investigated the activity of an extract of Rhus chinensis (RC) using metabolic activity and cytokine production of the BMs treated with LPS and RC. The metabolic activity of BMs was measured using Cell Counting Kit-8® solution. RC decreased the metabolic activity of LPS-treated BMs. A viability assay using trypan blue solution demonstrated that RC marginally decreased the viability of LPS-treated BMs. Flow cytometry analysis revealed that RC decreased the mitochondrial membrane potential of BMs, regardless of LPS treatment. To investigate the anti-inflammatory activity of RC, we measured the production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 in BMs. LPS increased the production of both cytokines in BMs. Interestingly, RC induced a greater increase in IL-10 than TNF-alpha in LPS-treated BMs. Taken together, RC decreased metabolic activity and modulated the production of inflammation-related cytokines in LPS-treated BMs. These findings suggest that RC can be used as a medicinal herb with anti-inflammatory activity.
서 론
재료 및 방법
실험동물과 오배자추출물
골수세포의 분리와 물질처리
골수세포의 대사활성도 측정
Cell morphology
유세포분석
Enzyme-linked immunosorbent assay (ELISA)
통계분석
결 과
골수세포의 대사활성도와 생존율에 대한 오배자의 효과
오배자를 처리 한 골수세포의 형태 변화
유세포분석을 통한 오배자가 처리된 골수세포의 크기변화 측정
오배자에 의한 골수세포의 염증성싸이토카인 생산 변화
오배자에 의한 골수세포의 미토콘드리아 막전위 저하
고 찰
REFERENCES
