This study was conducted to examine the effect of new inoculants on in vitro digestibility and fermentation characteristics of high moisture rye silage. Rye was harvested at heading stage and divided into 5 treatments, following: No additives(CON); L. plantarum R48-27(NI1); L. buchneri R4-26(NI2); mixture of NI1 and NI2 at 1:1 ratio(MIX); and L. buchneri(LB). The rye forage was ensiled into 10 L bucket silo for 100 days. In vitro digestibility of dry matter and neutral detergent fiber were highest(p<0.05) in NI2 silage. The pH in NI2 and LB silages were lower(p<0.05) than CON silage. Lactate concentration was highest(p<0.05) in NI1 silage. While concentrations of acetate and propionate were highest(p<0.05) in MIX silage. Lactates : acetate ratio was highest(p<0.05) in NI1 silage, but lowest in LB silage. Butyrate concentrations of NI2 and LB silages were lower(p<0.05) than that in CON and NI1 silages. Lactic acid bacteria (LAB) count in all inoculated silages was higher(p<0.05) than that in CON silage, while yeast count in LB silage was lower than in CON, NI1, and MIX silages. In conclusion, application of NI2 inoculant could improve potentially fermentation quality and digestibility of high moisture rye silage.

ABSTRACT
 Introduction
 Materials and methods
  1 Silage production
  2 Chemical compositions and in vitro digestibility
  3 Fermentation indices
  4 Microbial enumeration
  5 Statistical analysis
 Results
 Discussion
 References

• Seong Shin Lee(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
• Dimas H. V. Paradhipta(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
• Young Ho Joo(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
• Hyuk Jun Lee(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
• Hyun Son(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University)
• Ouk Kyu Han(Central Area Crop Breeding Division, National Institute of Crop Science, RDA)
• Dong Hyeon Kim(Department of Animal Sciences, IFAS, University of Florida)
• Sam Churl Kim(Division of Applied Life Science(BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Department of Animal Sciences, IFAS, University of Florida) Corresponding author