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FISH 기법을 이용한 생물활성탄 공정에서의 운전기간별 부착 박테리아 군집변화 분석 KCI 등재

Analysis of Attached Bacterial Community of Biological Activated Carbon Process Using FISH

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한국환경과학회지 (Journal of Environmental Science International)
한국환경과학회 (The Korean Environmental Sciences Society)
초록

The concentration of organic compounds was analyzed at each step of BAC process though BDOCtotal/rapid/slow. Further, bacteria communities and biomass concentrations measured FISH and ATP methods were analyzed. The bed volume (BV) of steady state is different from that of based on assessment of organic compounds removal. Bed volumes in DOC, BDOCrapid and BDOCtotal/slow removal at steady state were around 27,500 (185.8 day), 15,000 (101.4 day) and 32,000 (216.2 day), respectively. A biomass didn't change after the bed volume reached 22,500 (152.0 day) according to analyzing ATP concentration of bacteria. The concentration of ATP was 2.14 μg/g in BV 22,500 (152.0 day). The total bacterial number was 4.01±0.4×107 cells/g at the bed volume 1,150 (7.8 day) (the initial operation) and the number of bacteria was 9.27±0.2×109 at the bed volume 58,560 395.7 day) that increased more than 200 times. Bacterial uptrend was reduced and bacterial communities were stabilized since BV 18,720 (126.5 day). When BV were 1,150 (7.8 day), 8,916 (60.2 day), 18,720 (126.5 day), 31,005 (209.5 day), 49,632 (335.3 day), 58,560 (395.7 day), a proportion of total bacteria for the Eubacteria were 60.1%, 66.0%, 78.4%, 82.0%, 81.3% respectively. γ-Proteobacteria group was the most population throughout the entire range. The correlation coefficient (r2) between Eubacteria biomass and ATP concentration was 0.9448.

목차
Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 실험방법
 3. 결과 및 고찰
  3.1. 운전기간 증가에 따른 BDOC 제거 특성
  3.2. 운전기간 증가에 따른 활성탄 부착 박테리아 biomass평가
  3.3. FISH법을 이용한 운전기간 증가에 따른 부착 박테리아 군집 분석
 4. 결 론
 참 고 문 헌
저자
  • 손형식(부산대학교 미생물학과) | Hyeng-Sik Son (Department of Microbiology, Pusan National University)
  • 이상준(부산대학교 미생물학과) | Sang-Joon Lee (Department of Microbiology, Pusan National University) Corresponding author
  • 손희종(부산광역시 상수도사업본부 수질연구소) | Hee-Jong Son (Water Quality Institute, Waterworks Headquarter)
  • 박근태(부산대학교 산학협력단) | Geun-Tae Park (Research & University-Industry Cooperation, Pusan National University)