This study was designed to compare re-epithelization rate/feature of conjunctival epithelial cell on amniotic membrane and anatomical difference between normal amniotic membrane and de-epithelial amniotic membrane. Human conjunctival tissue obtained while cataract operation were used in this study. Primary cultured human conjunctival epithelial cell and fibroblast were incubated on intact amniotic membrane and de-epithelialized amniotic membrane. After incubation for 3, 5 and 7 days, re-epithelization rate was analyzed using cell tracker and microscopic feature was examined using methylene blue and hematoxylin- eosin stain. For cell marker analysis, cytokeratin 14 and vimentin antibody immunofluorescence stain were used. Clearance rate of epithelial cells on amniotic membrane using trypsin was better than using 25% alcohol. The results of reepithelization rate using cell tracker, HaCaT, conjunctival firbroblast, and epithelial cell were rapidly incubated on deepithelialized amniotic membrane. Wound healing and re-epithelization were more rapidly induced on de-epithelialized amniotic membrane on permanent amniotic membrane graft.

서 론
 대상 및 방법
  인체 결막조직 채취
  인체 결막 상피 및 섬유모세포 배양
  HaCaT 세포 배양
  양막 상피 제거
  세포배양을 위한 양막 처치
  Cell tracker를 이용한 세포추적
  냉동절편제작
  메틸렌 블루 염색
  헤마톡실린-에오신 염색
  면역조직화학염색(Immunohistochemistry)
 결 과
  양막의 면역조직화학적 분석
  양막의 상피 제거
  인체 결막 세포의 일차배양
  양막 상피 제거가 HaCaT 세포의 증식에 미치는 효과
  양막 상피 제거가 인체 결막 섬유모세포의 증식에 미치는 효과
  양막 상피 제거가 인체 결막 상피세포의 증식에 미치는 효과
  양막 위에 배양한 인체 결막 상피세포의 면역조직화학적 분석
 고 찰
 참고문헌

• 김용욱(동아대학교 의과대학 안과학 교실) | Long yu Jin (Department of Ophthalmology, Dong-A University College of Medicine, Busan, Korea)
• 민지상(동아대학교 의과대학 안과학 교실) | Ji Sang Min (Department of Ophthalmology, Dong-A University College of Medicine, Busan, Korea) Corresponding author
• 정우진(동아대학교 의과대학 안과학 교실) | Woo Jin Jung (Department of Ophthalmology, Dong-A University College of Medicine, Busan, Korea)
• 박우찬(동아대학교 의과대학 안과학 교실) | Woo Chan Park (Department of Ophthalmology, Dong-A University College of Medicine, Busan, Korea)