본 연구는 2013년부터 2017년까지 경남 한산·거제만 해역에서 생산되는 패류에 대한 위생지표세균, 마비성 패 류독소, 설사성 패류독소를 분석하여 세균학적·독물학적 안전성을 평가하였다. 굴 시료 404점에 대한 분변계대장균은 < 18~330 MPN/100 g의 범위를 나타내었으나, 대장 균 230 E. coli MPN/100 g을 초과한 시료는 없었다. 굴 수확시기 동안 분석한 대장균의 기하학적 평균치는 24.3 MPN/100 g으로 매우 낮게 나타났다. 한산·거제만해역에서 생산되는 굴의 세균학적 안전성을 평가한 결과, 식품위 생법의 생식용 굴에 대한 위생기준을 만족하였고, EU의 패 류생산해역 A등급 기준에 부합하였다. 한산·거제만해역 의 독물학적 평가를 위해 굴 532개 시료와 패류독소 지표 종인 지중해담치 268개 시료에 대한 분석을 실시하였다. 마비성패류독소는 2013년 4월에 지중해담치 3개 시료에서 1.20~2.29 mg/kg 범위로 기준을 초과하여 검출되었으나, 굴 시료에서는 전혀 검출되지 않았다. 총 120개 시료에서 설사성패류독소를 분석하였으나, 정량한계 이하로 매우 낮게 나타났다. 독물학적 안전성 평가결과, 굴 시료에서는 마비성 및 설사성패류독소가 전혀 검출되지 않았으나, 지 표종인 지중해담치에서는 마비성패류독소 기준치를 초과 한 것으로 나타나 지속적인 모니터링과 안전관리가 요구 되었다.

To evaluate bacteriological and toxicological safety hygienic indicator bacterium and paralytic and diarrhetic shellfish toxins in the shellfish produced in Hansan·Geojeman 2013-2017 were investigated. Fecal coliforms were < 18~330 MPN/100 g in 404 oyster samples. But all samples tested, did not exceed 230 E. coli MPN/ 100 g. Geometric mean of E. coli for oyster samples collected during major shellfish production period was 24.3 MPN/100 g, considerde stable results. Bacteriological quality of oysters collected from Hansan·Geojeman meets the standard value based on shellfish hygiene of the Food Sanitation Act of Korea and also meets Grade A, according to classification of shellfish harvesting areas of the European Union. For toxicological evaluation of Hansan·Geojeman, 532 oyster samples and 268 mussel samples as an indicator, were analyzed. Paralytic shellfish toxins were detected in the range of 0.42~2.29 mg/kg in eight mussel samples, and exceeded criteria in three samples from early to late April 2013. Diarrhetic shellfish toxin was detected in three of 120 samples, but it was revealed to be under regulation value (0.16 mg Okadaic Acid equ./kg). As a result of toxicological evaluation, paralytic and diarrhetic shellfish toxins were not detected in oyster samples, but it was found that mussel as an indicator species, exceeded the threshold value of paralytic shellfish toxin. Accordingly, sanitary surveys were continuously requested for food safety management of shellfish.

ABSTRACT
 Materials and Methods
  조사지점 및 시료의 채취
  위생지표세균 분석
  마비성패류독소 분석
  설사성패류독소 분석
 Results and Discussion
  세균학적 안전성평가
  수확시기에 따른 굴의 위생학적 성상
  강우가 굴의 세균학적 수질에 미치는 영향
  독물학적 안전성 평가
 국문요약
 References

• 하광수(국립수산과학원 식품위생가공과) | Kwang-Soo Ha (Food Safety and Processing Research Division, National Institute Fisheries Science) Correspondence to
• 이가정(남동해수산연구소) | Ka-Jeong Lee (Southeast Sea Fisheries Research Institute, National Institute Fisheries Science)
• 정연중(남동해수산연구소) | Yeon-Jung Jeong (Southeast Sea Fisheries Research Institute, National Institute Fisheries Science)
• 목종수(국립수산과학원 식품위생가공과) | Jong-Soo Mok (Food Safety and Processing Research Division, National Institute Fisheries Science)
• 김풍호(서해수산연구소) | Poong-Ho Kim (West Sea Fisheries Research Institute, National Institute Fisheries Science)
• 김연계(국립수산과학원 식품위생가공과) | Yeon-Kye Kim (Food Safety and Processing Research Division, National Institute Fisheries Science)
• 이희정(국립수산과학원 식품위생가공과) | Hee-Jung Lee (Food Safety and Processing Research Division, National Institute Fisheries Science)
• 김동욱(국립수산과학원 식품위생가공과) | Dong-Wook Kim (Food Safety and Processing Research Division, National Institute Fisheries Science)
• 손광태(국립수산과학원 식품위생가공과) | Kwang-Tae Son (Food Safety and Processing Research Division, National Institute Fisheries Science)