Oriental medicine uses many herbs with biological activity. Among these, some have anti-inflammatory activities, but their action mechanisms have yet to be fully elucidated. Therefore, in this study, we harvested mouse bone marrow cells (BMs) and treated them with lipopolysaccharide (LPS), after which they were treated with the extracts of four medicinal herbs. The metabolic activity, cell death ratio, and tumor necrosis factor (TNF)-alpha production of the BMs was then evaluated. Among the four medicinal herbs, Caesalpinia sappan (CS) significantly decreased the metabolic activity, mitochondrial membrane potential of BMs, and production of TNF-alpha, especially in the presence of LPS. Moreover, CS increased the cell death of BMs stained with propidium iodide. Taken together, these findings indicate that CS inhibited the biological activities of LPS-treated BMs in multiple assays, confirming that CS has anti-inflammatory activity.

Abstract
 서 론
 재료 및 방법
  실험동물과 한약제추출물
  골수세포의 분리와 물질처리
  골수세포의 대사활성도 측정
  유세포분석
  Enzyme-linked immunosorbent assay (ELISA)
  통계분석
 결 과
  골수세포의 대사활성도에 대한 한약재추출물의 효과
  골수세포의 TNF-alpha 생산 변화
  골수세포의 미토콘드리아 막전위 변화
  추출물에 의한 골수세포의 세포 생존율 변화
 고 찰
 REFERENCES

• So-Yeon Ahn(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
• Hong-Gu Joo(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University) Corresponding Author