Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were 25℃ and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 표준시약 및 배지
  2.2. Biphenyl 분해 미생물의 분리
  2.3. 고농도 biphenyl의 분해능 비교
  2.4. 균주의 생리적 특성 및 동정
 3. 결과 및 고찰
  3.1. Biphenyl 분해 미생물의 분리
  3.2. 분리 미생물들의 biphenyl 분해능
  3.3. 우수 균주의 생리적 특성 및 동정
 4. 결 론
 참 고 문 헌

• 이대성(포항공과대학교 해양대학원) | Dae-Sung Lee (POSTECH Ocean Science & Technology Institute, POSTECH)
• 정성윤(대구가톨릭대학교 의생명과학과) | Seong-Yun Jeong (Department of Medical Life Science, Catholic University of Daegu) Corresponding Author