This study was carried out to compare the toxicity of nano and micrometer particles with Cu and Zn on soil microbial community and metal uptake of buck wheat. In microcosm system, soil was incubated for 14 days after soil aliquots were artificially contaminated with 1,000 mg/kg Cu, Zn nano and micro particles, respectively. After then, buck wheat was planted in incubating soils and non incubating soils. After 14 days, we compared bioaccumulation of metal, and microbial carbon substrate utilization patterns between incubating soils and non-incubating soils. The enrichment factor (EF) values of incubating samples were greater than non-incubating soils. Dehydrogenase activity had been inhibited by Cu and Zn nanoparticles in non-incubating soil, as well as it had been inhibited by Zn micro particles in incubating soils. Results of biolog test, it was not significant different between nano particles and micro particles. It cannot be generalized that nanoparticles of metal are always more toxic to soil microbial activity and diversity than micrometer-sized particles and the toxicity needs to be assessed on a case-by-case basis.

Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 토양 마이크로코즘 실험 준비 및 시료 채취
  2.3. 중금속 분석
  2.4. Dehydrogenase activity (DHA) 분석
  2.5. 기질 이용도 분석
  2.6. 통계분석
 3. 결과 및 고찰
  3.1. 금속 입자의 특성
  3.2. 금속 입자의 생물 축적
  3.3. 토양의 dehydrogenase(DHA) 활성
  3.4. 기질 이용도
 4. 결 론
 참 고 문 헌

• 김성현(연세대학교 토목환경공학과) | Sunghyun Kim (School of Environmental Engineering, Yonsei University)
• 김정은(이화여자대학교 분자생명과학부) | Jungeun Kim (Department of Life Science, Ewha Womans University)
• 곽영지(이화여자대학교 분자생명과학부) | Youngji Gwak (Department of Life Science, Ewha Womans University)
• 김연지(이화여자대학교 분자생명과학부) | Yunji Kim (Department of Life Science, Ewha Womans University)
• 이인숙(이화여자대학교 에코과학부) | Insook Lee (Division of Eco Science, Ewha Womans University) Corresponding Author