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국내 양돈장의 사료 내 아플라톡신 오염도 조사 KCI 등재

Survey on aflatoxins in pig feeds in Korean pig farms

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

Aflatoxins produced by Aspergillus spp. are recognized as a major concern in animal and human health. In pigs, ingestion of aflatoxin-contaminated feeds causes immunosuppression, hepatotoxicosis and poor feed efficiency. In this study, we screened the contaminated level of aflatoxins in 449 pig feeds from 12 swine farms in Korea. For rapid and efficient screening of aflatoxins in pig feeds we evaluated the feasibility of three commercial ELISA kits for the screening of aflatoxins in pig feeds. Twenty-nine pig feed samples were examined for total aflatoxins using three ELISA kits, simultaneously. From three repetitions of each assay, the average intra-assay precisions and the average inter-assay precisions expressed as coefficient of variation (CV, %) for VeratoxⓇ Quantitative Aflatoxin test were 6.90 and 12.29, respectively. A statistical comparison of the results between HPLC and ELISAs showed that the correlation coefficient values for VeratoxⓇ was 0.96. The results demonstrated that we can apply the VeratoxⓇ Quantitative Aflatoxin test for the detection of aflatoxins in pig feed from the field. The screening of field samples with this ELISA kit showed that 11 out of 265 pig feeds for growers and weaners were contaminated with total aflatoxin levels exceeding 10 ppb, maximum tolerable limits for their compound feeds and the aflatoxins levels of remaining 184 pig feeds for other age groups of pigs were confirmed as below 20 ppb. The results from the screening indicated overall low levels of aflatoxins contamination in pig feeds.

목차
ABSTRACT
 서 론
 재료 및 방법
  1. 아플라톡신 검사방법
  2. 돼지농장 사료 내 아플라톡신 오염도 조사
 결 과
  1. 아플라톡신 검사 킷트간 비교 시험
  2. 돼지 농장 내 사료에 대한 아플라톡신 오염도 분석
 고 찰
 참고문헌
저자
  • 송윤경(국립수의과학검역원) | Yun-Kyung Song (National Veterinary Research and Quarantine Service)
  • 정병열(국립수의과학검역원) | Byeong-Yeal Jung (National Veterinary Research and Quarantine Service)
  • 최정수(국립수의과학검역원) | Jeong-Soo Choi (National Veterinary Research and Quarantine Service)
  • 문운경(국립수의과학검역원) | Oun-Kyung Moon (National Veterinary Research and Quarantine Service)
  • 이수형(국립농업과학원 농산물안전성부) | Soo-Hyung Lee (National Institute of Agicultural Science and Technology)
  • 정덕화(경상대학교 농생명학부) | Duck-Hwa Chung (Division of Applied Life Science, Gyeongsang National University)
  • 박선일(강원대학교 수의과대학) | Son-Il PAK (Department of Veterinary Medicine, Kangwon National University)
  • 박최규(국립수의과학검역원) | Choi-Kyu Park (National Veterinary Research and Quarantine Service) Correspondence