A simple, selective and sensitive procedure for the confirmation of 14 sulfonamide antibacterials in milk was developed. The milk samples were homogenized, extracted and deproteinized by acetonitrile and defatted by n-hexane. Analysis was performed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) in positive mode for all 14 analytes. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. The calibrations were performed in sample matrixes and the interference effect of sample matrixes on the ionization was effectively eliminated. Good linear relationship (R2=0.992～ 0.999) were observed at 6 concentrations of 2.5～100 ng/g. Satisfied recoveries (86.3～110.2%) of all sulfonamides were demonstrated in spiked milk at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for sulfonamides ranged from 0.25～2.1 ng/g.

ABSTRACT
 서 론
 재료 및 방법
  1. 표준물질 및 시약
  2. 분석기기 및 분석조건
  3. 표준 용액 조제
  4. 표준검량선 작성
  5. 시료 전처리
  6. 분석법 검증
 결과 및 고찰
  1. LC-MS/MS 분석조건 확립
  2. 분석법 검증
 참고문헌

• 임채미(국립수의과학검역원) | Chae-mi Lim (National Veterinary Research and Quarantine Service) Correspondence
• 권현정(국립수의과학검역원) | Hyun-Jeong Kwon (National Veterinary Research and Quarantine Service)
• 조병훈(국립수의과학검역원) | Byung-hoon Cho (National Veterinary Research and Quarantine Service)
• 박수정(국립수의과학검역원) | Su-jeong Park (National Veterinary Research and Quarantine Service)
• 정갑수(국립수의과학검역원) | Gap-su Chung (National Veterinary Research and Quarantine Service)
• 손성완(국립수의과학검역원) | Seong-wan Son (National Veterinary Research and Quarantine Service)