Vanadium, a dietary micronutrient, has been reported to present interesting biological and pharmacological properties, including superoxide and nitric oxide scavenging effects. Low-dose ionizing radiation (LDR) is known to damage DNA and cause apoptosis of peripheral immunocytes by producing reactive oxygen species (ROS). The aim of this study was to elucidate the capacity of immune activation of Jeju water containing vanadium on immunosuppression caused by LDR. We examined the ROS production, DNA damage, cell apoptosis and proliferation of peripheral immunocytes in irradiated mice drinking different concentrations for 90 days; V0 (vanadium 0㎍/L, control), V1 (vanadium 15～20㎍/ L) and V2 (vanadium 20∼25㎍/L). Compared to V0 control where level of ROS showed tendency to increase, the ROS production was attenuated in peripheral immunocytes of irradiated mice drinking V1 and V2. DNA damage of peripheral immunocytes triggered by LDR significantly increased in mice drinking V0 compared to non-irradiated control, whereas V1 and V2 dramatically induced remission of DNA damage. On the observation of apoptosis of peripheral immunocytes, V1 and V2 showed the potency to reduce the number of apoptotic cells. On the other hand irradiated mice drinking V0 exhibited raised number of apoptotic cells. From the results obtained, we speculated that Jeju water containing vanadium (V1 and V2) has a potential role in decreasing DNA damage and apoptosis of immune cell by inhibiting ROS production. Consistent with this, Jeju water containing vanadium (V1 and V2) exhibits a capacity to enhance cell proliferation of peripheral immunocytes, which is suppressed by LDR as shown in V0 control. Collectively, Jeju water containing vanadium reduced DNA damage and apoptosis and induced the stimulatory potential on immunocytes. These results suggest that Jeju water containing vanadium sustained immune activities under immunosuppression caused by LDR.

ABSTRACT
 서 론
 재료 및 방법
  1. 실험 물질
  2. 실험 동물
  3. 저선량의 방사선 조사에 의한 면역기능 억제 동물모델구축
  4. 마우스의 비장세포 부유액 준비
  5. Fluorescence spectrophotometer를 이용한 세포내 ROS측정
  6. Comet assay를 이용한 말초면역세포에서의 DNA 손상 측정
  7. Flow cytometry를 이용한 apoptotic sub-G1 hypodiploid측정
  8. 말초면역세포의 증식능 검사
  9. 유세포분석 (Flow cytometry)을 통한 말초면역세포(T 세포, B 세포)의 수적 변화 검사
  10. 통계처리
 결 과
  1. 바나듐수를 음수시킨 마우스의 말초면역세포 내ROS 감소
  2. 바나듐수를 음수시킨 마우스의 말초면역세포에서의DNA 손상 억제
  3. 바나듐수를 음수시킨 마우스 말초면역세포의 apoptoticsub-G1 hypodiploid 억제
  4. 바나듐수를 음수시킨 면역기능 억제 동물모델의 말초면역세포 증식능 증가
  5. 바나듐수를 음수시킨 면역기능 억제 동물모델의 말초면역세포 집단의 변화
 고 찰
 참고문헌

• 하단비(제주대학교 수의과대학 수의학과) | Danbee Ha (College of Veterinary Medicine, Jeju National University)
• 김민주(한국기초과학지원연구원 해양바이오연구팀) | Min Ju Kim (Marine Bio Research Team, Korea Basic Science Institute (KBSI))
• 주해진(제주대학교 수의과대학 수의학과) | Haejin Joo (College of Veterinary Medicine, Jeju National University)
• 조진희(제주대학교 수의과대학 수의학과) | Jinhee Cho (College of Veterinary Medicine, Jeju National University)
• 빙소진(제주대학교 수의과대학 수의학과) | So Jin Bing (College of Veterinary Medicine, Jeju National University)
• 임윤규(제주대학교 수의과대학 수의학과) | Yoon-Kyu Lim (College of Veterinary Medicine, Jeju National University)
• 현진원(제주대학교 의과대학 의학과) | Jin Won Hyun (School of Medicine, Jeju National University)
• 지영흔(제주대학교 수의과대학 수의학과) | Youngheun Jee (College of Veterinary Medicine, Jeju National University) Correspondence