A rapid, simple and reliable LC-MS/MS method, which can be used on a routine basis, was developed for the simultaneous detection of 8 penicillin antibiotics (amoxicillin, ampicillin, penicillin-G, penicillin-V, oxacillin, cloxacillin, nafcillin and dicloxacillin) in swine muscle and kidney. The antibiotics were extracted from samples with water and methanol. The extract was centrifuged, filtered and analyzed by liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS), using a C18 reversed phase column with water/acetonitrile gradient containing 0.05 % formic acid. Mass spectral acquisition was achieved in an electrospray positive ion mode by applying multiple reaction monitoring (MRM) of 2 fragment ion transitions to provide a high degree of sensitivity and specificity. The ion rations were consistent and could be used for confirmation of identity of the penicillin antibiotics. Recoveries of eight penicillins at three fortification levels (10, 20 and 40㎍/㎏) ranged from 79.8 to 102.4% and 72.8 to 103.4% in swine muscle and kidney, respectively. The coefficient of variation was than 9% in all samples. The estimated limits of quantification (LOQs) ranged from 1.0 to 3.2㎍/㎏ in swine muscle and kidney, respectively. The LOQs of this method are below the MRLs of penicillin antibiotics in animal tissues established in Korea and other countries.

ABSTRACT
 서 론
 재료 및 방법
  1. 표준품 및 시약
  2. 분석기기 및 분석조건
  3. 표준용액 조제
  4. 시료 첨가 표준곡선 작성
  5. 시료 전처리법
  6. 분석법 검증
 결과 및 고찰
  1. LC-MS/MS 분석조건 확립
  2. 시료전처리
  3. 분석법 검증
 참고문헌

• 임채미(농림수산검역검사본부 독성화학과) | Chae-mi Lim (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency) Correspondence
• 권현정(농림수산검역검사본부 독성화학과) | Hyun-Jeong Kwon (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
• 조병훈(농림수산검역검사본부 독성화학과) | Byung-hoon Cho (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
• 박수정(농림수산검역검사본부 독성화학과) | Su-jeong Park (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
• 정갑수(농림수산검역검사본부 독성화학과) | Gap-su Chung (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)
• 손성완(농림수산검역검사본부 독성화학과) | Seong-wan Son (Toxicology and Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency)