During 2008 2010, 943 swine sera were collected from 45 farms located nationwide. Antibodies against porcine epidemic diarrhea virus (PEDV) were tested via serum neutralization antibody test (SNT) using PEDV-SN, which was adapted and propagated on the Vero cell monolayer with trypsin-free culture media supplemented with more than 10% fetal bovine serum (FBS). All 45 farms were shown to have at least one or more seropositive pig. Of the 943 swine sera that were tested, 931 sera were neutralizing antibody positive against PEDV. These high seroprevalence rates seemed to be due to vaccination or natural infection of PEDV. In a plaque reduction neutralization test (PRNT) using a swine serum showing SN titer of 1:32, a greater than 50% plaque reduction was observed in up to 160 times serum dilution.

ABSTRACT
 서 론
 재료 및 방법
  1. 세포
  2. 바이러스
  3. 검사시료
  4. 중화항체 시험
  5. Plaque reduction neutralization test (PRNT)
 결과 및 고찰
 참고문헌

• Yeon-Joo Kim(College of Veterinary Medicine, Chungnam National University)
• Sang-Hoon Park(Seoul Metropolitan Government Research Institute of Public Healthe and Environment)
• Sang-Heui Seo(College of Veterinary Medicine, Chungnam National University)
• Hyun-Soo Kim(College of Veterinary Medicine, Chungnam National University) Correspondence