조류 소재에 관한 연구는 꾸준히 진행되고 있다. 본 연구에서는 미역, 다시마, 불등가사리 등 해조류를 혼합 한 발효추출물의 항산화, 미백, 보습의 활성 실험을 진행하였다. 또한 유산균으로는 김치유산균 Lactobacillus sakei균주를 이용하였다. 복합해조류 발효추출물의 생리활성 실험을 진행하였으며, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) 라디칼 소거능에서는 양성대조군 보다 높은 저해효능을 보였다. 미백효능 실험에서는 3,4-dihydroxy-L-phenylalanine (L-DOPA), mushroom tyrosinase 실험을 수행하였으며, 발효 전보다 발효 후의 미백활성이 높게 나타나는 것을 확인할 수 있었다. 한편, 해조류 소재의 유효성분인 fucose에 대한 HPLC분석을 수행하였으며, 용매조건에 대한 표준화 방법을 새로이 정립하였다. 이 연구는 복합해조류 추출물이 항산화, 미백 등 화장품 소재로서 가능성을 제안할 수 있다.

Studies on seaweed-based materials have been progressing steadily day by day. In this experiment, we checked the anti-oxidant, whitening, and moisturizing activities of fermented extract from a mixture of Undariapinnatifida, Saccharina japonica, and Gloiopeltis furcate. Also, Lactobacillus sakei strains of kimchi were used as the lactic acid bacteria. The physiological status of the combined seaweed extracts was also investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging results showed that the inhibitory effects of the combined seaweed extracts were higher than the positive control. Furthermore, 3,4-dihydroxy- L-phenylalanine (L-DOPA) and Mushroom tyrosinase tests were conducted during the whitening efficacy experiment. Hence, it was confirmed that the whitening activity of fermented extracts was greater than the extracts without fermentation. HPLC analysis of fucose (an active ingredient of seaweed) was also performed and a standard method for solvent conditions was newly established. This study suggests that the composite of algae extract has potentials to be used as anti-oxidant and whitening agents in cosmetics.

요 약
 Abstract
 1. 서 론
 2. 재료 및 방법
  2.1. 실험재료
  2.2. 기기 및 시약
  2.3. 추출 및 발효조건
  2.4. 복합해조류 발효추출물의 항산화 효능 측정
  2.5. 복합해조류 발효추출물의 미백활성 측정
  2.6. Hyaluronic Acid 함량측정
  2.7. 복합해조류 발효추출물의 세포 생존율 측정
  2.8. 복합해조류 발효추출물의 유효성분 분석
 3. 결과 및 고찰
  3.1. 복합해조류 발효추출물의 항산화 효능 측정
  3.2. 복합해조류 발효추출물의 미백 효능 측정
  3.3. 복합해조류 발효추출물의 보습 효능 측정
  3.4. 복합해조류 발효추출물의 세포 생존율
  3.5. 복합해조류 발효추출물의 유효성분 TLC 및 HPLC 분석
 4. 결 론
 Reference

• 강세원(월드코스텍, 지역주도형R&D) | Se-Won Kang (Research Institute, World Costec, Co. Ltd)
• 김은지(월드코스텍, 지역주도형R&D) | Eun Ji Kim (Research Institute, World Costec, Co. Ltd)
• 정유린(월드코스텍, 지역주도형R&D) | Yu-Rin Jung (Research Institute, World Costec, Co. Ltd)
• 고해주(월드코스텍, 지역주도형R&D) | Hae Ju Ko (Research Institute, World Costec, Co. Ltd)