Background: To enhance the taste and physiological characteristics of Lycii fructus (Gugija) extracts, we investigated the changes in the physiological characteristics of Gugija extracts caused by adding white ginseng (WG) and red ginseng (RG)
Methods and Results: Gugija extracts, including 10G10, 10GW-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixtures made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with WG), as well as 10G10, 10GR-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixture made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with RG) were extracted with water at 10 times the respective mixture's volume. The antioxidant activities of Gugija extracts were investigated by assessing their 1,1-diphenyl-2-picrydrazyl (DPPH) and 2,2’-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant potential (FRAP) activity, nitrite scavenging activity, and angiotensin converting enzyme (ACE) inhibitory activity. As the amount of WG added increased, the DPPH, and, ABTS radical scavenging activity, and FRAP activity of the Gugija extract decreased. The half maximal inhibitory concentration (IC50) value of 10G10, 10GW-G6 : 4, 10GR-G6 : 4, and 10GR-G0 for DPPH radical scavenging activity were 25.50 ± 1.04, 52.06 ± 1.46, 16.87 ± 1.24, and 9.50 ± 0.16 ㎕/㎖, respectively. On the other hand, the physiological activity of Gugija extract increased with the addition of increasing amounts of RG. However, ACE inhibitory activity was the highest (50.25 ± 2.58%) in the Gugija 10-fold extract without any added RG.
Conclusions: From the above results, we suggest that adding RG to Gugija extracts increase their antioxidant, FRAP, and nitrite scavenging activities.

ABSTRACT
 서 언
 재료 및 방법
  1. 실험재료
  2. 추출물의 수율 및 당도
  3. DPPH radical 소거능 측정
  4. ABTS radical 소거능 측정
  5. Ferric reducing antioxidant power (FRAP) 측정
  6. 아질산염 소거능
  7. Angiotensin-converting enzyme (ACE) 저해활성
  8. 추출물의 베타인 및 사포닌 함량
  9. 추출물의 총 폴리페놀 함량
  10. 통계처리
 결과 및 고찰
  1. 추출물의 수율 및 당도
  2. DPPH radical 소거능
  3. ABTS radical 소거능
  4. Ferric reducing antioxidant power (FRAP)
  5. 아질산염 소거능
  6. Angiotensin-converting enzyme (ACE) 저해활성
  7. 추출물의 베타인, 진세노사이드 및 총폴리페놀 함량
  8.베타인, 총진세노사이드 및 총폴리페놀함량과 생리기능성간의 상관관계
 REFERENCES

• 성봉재(충남농업기술원 인삼약초연구소) | Bong Jae Seong (Ginseng and Medicinal Plant Research Institute, CNARES)
• 김선익(충남농업기술원 인삼약초연구소) | Sun Ick Kim (Ginseng and Medicinal Plant Research Institute, CNARES)
• 지무근(충남농업기술원 인삼약초연구소) | Moo Geun Jee (Ginseng and Medicinal Plant Research Institute, CNARES)
• 김수동(충남농업기술원 인삼약초연구소) | Soo Dong Kim (Ginseng and Medicinal Plant Research Institute, CNARES)
• 권아름(충남농업기술원 인삼약초연구소) | A Reum Kwon (Ginseng and Medicinal Plant Research Institute, CNARES)
• 김현호(충남농업기술원 인삼약초연구소) | Hyun Ho Kim (Ginseng and Medicinal Plant Research Institute, CNARES)
• 원준연(중부대학교 한방건강관리학과) | Jun Yeon Won (Department of Oriental healthcare, Joongbu University)
• 이가순(충남농업기술원 인삼약초연구소) | Ka Soon Lee (Ginseng and Medicinal Plant Research Institute, CNARES) Corresponding author