Hydrogen gas is used as a fuel for the proton exchange membrane fuel cell (PEMFC). Trace amount of carbon monoxide present in the reformate H₂ gas can poison the anode of the PEMFC. Therefore, preferential oxidation (PROX) of CO is essential for reducing the concentration of CO from a hydrogen-rich reformate gas. In this study, conventional Pt/Al₂O₃catalyst was prepared for the preferential oxidation of CO. The effects of catalyst preparation method, additive, and hydrogen on the performances of PROX reaction of CO were investigated. Water treatment and addition of Ce enhanced catalytic activity of the Pt/Al₂O₃ catalyst at low temperature below 100℃.