This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was 162 μmol/min/g protein. I50 for AChE by carbamate pesticide with wet kit was 0.169 mg/L of carbaryl and 0.089 mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at 4oC and 25℃ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05 mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multiresidue method to detect the carbamate pesticides.

• 김정호(대구한의대학교 소방방재환경학과) | Jung-Ho Kim Corresponding Author