In order to measure in-situ suspended particle size, volume concentration of suspended particulate matter and current speed, mooring observation was performed at the Gwangyang Bay by using of an optical instrument, 'LISST-100' and an acoustic instrument, 'ADV'(St. S1). And the sediment flux was obtained based on the concentration of suspended particulate matter and current speeds measured at three lines of Gwangyang Bay during ebb and flood tide of August 2006. To investigate the spatial variation of suspended particulate matter, profiling observations were measured difference echo intensity and beam attenuation coefficient by using of ADCP and Transmissometer (Line A, B, C). The suspended sediment flux rate at the mouth of Gwangyang Bay was observed to be higher during asymmetrical than symmetrical of current speeds. The flux of suspended particulate matter concentration and current speeds were transported to southeastern direction of surface layer and northwestern direction of bottom layer at the western area at line A of Gwangyang Bay. Small suspended particles have been found to increase attenuation and transmission more efficiently than similar large particles using acoustic intensity (ADV/ADCP) or optical transmit coefficient (LISST-100/Transmissometer). The application and problems as using optical or acoustic instruments will be detected for use in time varying calibrations to account for non-negligible changes in complex environments in situ particle dynamics are poorly understood.