The lack of seed sludges for Ammonium Oxidizing Bacteria (AOB) and slow-growing ANaerobic AMMonium OXidation (ANAMMOX) bacteria is one of the major problem for large-scale application. In this study, 24m3 of single-stage SBR (Sequencing Batch Reactor) was operated to remove nitrogen from reject water using AOB and ANAMMOX bacteria cultivated from activated sludge in the field. The ANAMMOX activity was found after 44 days of cultivation in the ANAMMOX cultivation reactor, and then 0.66 kg N/m3/d of the nitrogen removal rate was achieved at 0.78 kg N/m3/d of the nitrogen loading rate at 153 days of cultivation. The AOB cultivation reactor showed 0.2 kg N/m3/d of nitrite production rate at 0.4 kg N/m3/d of nitrogen loading rate after 36 days of operation. The cultivated ANAMMOX bacteria and AOB was mixed into the single-stage SBR. The feed distribution was applied to remove total nitrogen stably in the single-stage SBR. The nitrogen removal rate in the single-stage SBR was gradually enhanced with an increase of specific activities of both AOB and ANAMMOX bacteria by showing 0.49 kg N/m3/d of the nitrogen removal rate at 0.56 kg N/m3/d of the nitrogen loading rate at 54 days of operation.

ABSTRACT
 1. 서 론
 2. 실험재료 및 방법
  2.1 AOB 미생물 배양
  2.2 ANAMMOX 미생물 배양
  2.3 SBR 반응조 및 운전 전략
  2.4 실제 반류수 특성
  2.5. ANAMMOX 활성도 평가
  2.6. 분석 방법
 3. 결과 및 토론
  3.1. 아질산화 미생물 배양
  3.2 ANAMMOX 미생물 배양
  3.3 단일 반응조에서 부분 아질산화 및 ANAMMOX를통한 질소 제거능 평가
  3.4 단일 반응조에서의 슬러지 특성 및 활성 변화
 4. 결 론
 References

• 최대희(영남대학교, 환경공학과) | Daehee Choi (Yeungnam university, Department of environmental engineering)
• 진양오(두산중공업, Water 기술개발팀) | Yangoh Jin (Doosan Heavy Industries & Construction, Water Technology Development Team)
• 이철우(두산중공업, Water 기술개발팀) | Chulwoo Lee (Doosan Heavy Industries & Construction, Water Technology Development Team)
• 정진영(영남대학교, 환경공학과) | Jinyoung Jung (Yeungnam university, Department of environmental engineering) Corresponding author