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SHP2 is Indispensable for growth factors signal transduction during bovine oocyte maturation and blastocyst development
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/362119
The aim of this study was to investigate the role of Src homology 2-containing
phosphotyrosine phosphatase SHP2 in intricate signaling network invoked by oocyte to
achieve cytoplasmic maturation and also blastocyst development. Activation of SHP2
regulates multicellular differentiation, proliferation and survival through numerous signal
pathways. The most prominent pathway is RAS/PI3K and p-AKT signaling cascade, as
a result mitogenic effect become enhanced. Oocytes were cultured in cisplatin an
anticancer drug, but selective activator of SHP2 and our grouping were SOF medium alone,
SOF + EGF, SOF + CISPLATIN 0.3 μM, and SOF + EGF + CISPLATIN 0.3 μM. We
evaluated that EGF neutralizes the apoptotic effect of cisplatin as well as maintain the
high expression of SHP2, as a result blastocyst development become boosted up. We
also found that inhibition of SHP2 with its specific inhibitor PHPS1 5 μM decreases the
blastocyst development and neutralizes growth factors effect. The developmental ability
and quality of bovine embryos were determined by assessing their cell number, gene
expression, immunofluorescence, and immunoblot. The differences in embryo
development between experimental groups were analyzed by one-way ANOVA. Our
results show that SHP2 have significant effect on MAP kinase pathways which expand
the cumulus cells during oocyte maturation and blastocyst development as compare to
inhibition of SHP2 with PHPS1. SHP2 not only transduce the signaling of epidermal growth
factor but it also has a role in signal transduction of FGF and IGF. The expression of
ERK, PI3K/p-AKT and mTOR was increased with EGF, but with the treatment of SHP2
inhibitor the expression of these genes become drop done. So we can conclude from these
results that SHP2 is important for oocyte maturation as well as for blastocyst
development.
