Basic reproductive biology of the daggertooth pike conger, Muraenesox cinereus were investigated based on the samples from August 2015 to December 2017 in Korean waters. A total of 2,328 samples was collected (1,593 females and 735 males) during the study period. The mean anal lengths (AL) were 33.5 ± 9.2 cm in females and 26.2 ± 7.3 cm in males respectively. The females were larger than males in AL (p < 0.05). Sex ratio was 2.2:1 in female to male (p < 0.05). The gonadosomatic index (GSI) varied monthly. GSI of female reached the maximum in July 2016 and June 2017 and the minimum in October 2016 and September 2017 respectively. The spawning period was from June to October. The size at sexual maturity (L50) of females was estimated at 24.9 cm in AL (63.5 cm in TL).