The effects of zeatin on Cd2+ -induced physiological toxicities in Commelina communis L
Three-week old Commelina communis was transferred and grown in Hoagland solution containing 100μM Cd2+, 100μM Cd2++100μM kinetin, 100μM Cd2++100μM zeatin and 100μM Cd2++200μM zeatin for 7 days, and then a number of physiological activities were investigated. In control, the length of the stem of plants was increased to 4.7cm, but in Cd2+, Cd2++kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin treatments, the growth of plants were increased to 1.5cm, 2.1cm, 3.9cm and 4.3cm, respectively. In the treatments of Cd2+, Cd2+ +kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin, total chlorophyll contents were reduced to 26%, 24%, 15% and 3%, respectively, on the contrast to the control. In chlorophyll fluorescence experiments, Fv/Fm ratios were also reduced to 44%, 21%, 17% and 5% in the light intensity of 2100μmole E m-2s-1 by Cd2+, Cd2++ kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin treatments on the contrast to the control. Water stresses were increased to 2.6, 1.7 and 1.2 times by Cd2+, Cd2++kinetin and Cd2++100μM zeatin. On the other hand, combination of Cd2++200μM zeatin reduced water stress to 0.12%. In Cd2+ accumulation experiments Cd2+ transports were inhibited to 33%, 48% and 70% by Cd2++kinetin, Cd2++100μM zeatin and Cd2++200μM zeatin. Therefore, it could be concluded that zeatin clearly reduced the toxicities of Cd2+ by reducing the absorption of Cd2+.